Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies.
نویسندگان
چکیده
Histone modifications are central to epigenetic regulation and must be reestablished with each round of DNA replication. Here we describe methods to localize these modifications within mammalian nuclei and to relate them to specific spatiotemporal patterns of DNA replication.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 325 شماره
صفحات -
تاریخ انتشار 2006